Fig. 2

Association of ZnPc-EVs with cancer cells and dendritic cells. MC38 or D1DCs were incubated with a B16-ZnPc, b Milk-ZnPc, c M1-ZnPc and d M2-ZnPc for 0–6 h. Fluorescence of ZnPc was measured in the cells by flow cytometry. e Equal numbers of a co-culture of MC38CFP and D1DCs were incubated with the ZnPc-EVs for 4 h, after which the fluorescence of ZnPc was measured by flow cytometry. Separation of cell lines was based on gating for CFP+ (cancer cells) or CD11c+ (D1DCs). Statistical significance was determined by Students t-test (**P<0.01, *P<0.05; mean±SEM; n=3)