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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: M1-derived extracellular vesicles enhance photodynamic therapy and promote immunological memory in preclinical models of colon cancer

Fig. 3

ZnPc-M1 is toxic to cancer cells, but not to D1DCs in absence of light. a MC38 or b D1DCs were incubated with the ZnPc-EVs for 4 h and left in the dark (dark toxicity, dT), treated with 690 nm at 200 mW/cm2 for 25 J/cm2 or subjected to three cycles of freeze-thawing at − 20 °C (FT). At 18 h after treatment, the cells were stained for viability marker DAPI and analyzed by flow cytometry. A co-culture of MC38CFP and D1DCs was subjected to the same treatment in addition to antibody staining of CD11c, showing the viability of c MC38CFP or d D1DCs. Separation of cell lines was based on gating for CFP+ (cancer cells) or CD11c+ (D1DCs). (***P<0.001; **P<0.01; mean±SEM; n=3)

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