Fig. 7
From: Advances of nano drug delivery system for the theranostics of ischemic stroke
A Design of RITC-labelling and mPEG conjugation of PAMAM-NH2. B Fluorescent confocal microscopy images showing bEnd.3/rat primary astrocytes in vitro BBB model in contact with PEGylated G4 PAMAMRITC dendrimers max intensity Z projections and orthogonal views of the in vitro BBB model layers at 24 h, after 4 h contact with (i) G4 PAMAMRITC, (ii) G4 PAMAMRITC-4mPEG, (iii) G4 PAMAMRITC-8mPEG and (iv) G4 PAMAMRITC-12mPEG is shown in red, the cell nuclei are stained with Hoechst (blue) and F-actin was stained with Alexa Fluor® 488 phalloidin. C Horizontal orthogonal views of the in vitro BBB model at 4 and 24 h after 4 h contact with PAMAMRITC conjugates or free RITC. Reproduced with permission from [110]. Copyright 2018, Elsevier