Fig. 9

Surface charge-dependent biodistribution of NMs. A schematic of illustration of fluorescently labelled liposomes and quantification in zebrafish. B liposomes distribution in kdrl:GFP transgenic embryos, 1 h p.i. C in vivo imaging of liposomes in circulation (measured in the lumen of the dorsal aorta, and liposome association with different blood vessel types. CHT-EC: caudal hematopoietic tissue endothelial cells, DLAV: dorsal longitudinal anastomotic vessel. ISV: intersegmental vessel. D liposomes distribution at tissue level. Quantification of liposome levels: E in circulation based on mean rhodamine fluorescence intensity in the lumen of the dorsal aorta. F associated with venous vs. arterial endothelial cells based on rhodamine fluorescence intensity associated with caudal vein (CV) vs. DA at 8 h p.i. G outside of the vasculature between the DLAV and DA at 8 h p.i. H associated with the vessel wall based on rhodamine fluorescence intensity associated with all endothelial cells relative to rhodamine fluorescence intensity in circulation at 1 h p.i. Reproduced with permission from [205]. Copyright 2018, American Chemical Society