Fig. 3
From: A novel inhalable quercetin-alginate nanogel as a promising therapy for acute lung injury
Cell viability, cell uptake and protective effect of QU-Nanogel on A549 cells or RAW264.7 cells. Cell viability of A549 cells treated with different drugs by MTS assay. (a) Incubated in different concentrations of PQ for 6 and 12 h. (b) Incubated in quercetin, QU-Nanogel, BLK-Nanogel and ulinastatin for 6 and 12 h. (**) denotes statistical significance in comparison to the control group (**p < 0.01). (c) The uptake of QU-Nanogel by RAW264.7 cells were analyzed by inverted fluorescence microscope. QU-Nanogel was labeled with rhodamine B isothiocyanate (RBITC) (Red fluorescence), DAPI (Blue fluorescence) and DiO (Green fluorescence) was used to stain the cell nuclei and cell menbrance. (d) The protective effects of quercetin and QU-Nanogel on PQ (600 µM) injured A549-GFP cells. The A549-GFP cells emitted bright green fluorescence in the state of cell survival. DiI (Red fluorescence) and DAPI (Blue fluorescence) were used to dye cell membrane and cell nucleus, respectively