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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Enhancement of antitumor immunotherapy using mitochondria-targeted cancer cell membrane-biomimetic MOF-mediated sonodynamic therapy and checkpoint blockade immunotherapy

Fig. 2

In vitro uptake and stimulation of in vitro immune response. a Homotypic effect of Zr-TCPP(TPP)/R837@M on four cell lines, namely 4T1, MDA-MB-468, Hepa1-6, and Bxpc-3, examined by flow cytometry. b Statistical analysis of four cell lines after 8 h incubation with Zr-TCPP(TPP)/R837@M or Zr-TCPP(TPP)/R837. c CLSM images to show subcellular localization of Zr-TCPP(TPP)/R837@M, and Zr-TCPP/R837@M. Scale bar: 25 μm. d Time related SOSG (5 μM) fluorescence changes of Zr-TCPP(TPP)/R837@M under US irradiation. e CLSM images of 4T1 cells after treated with of Zr-TCPP(TPP)/R837@M with or without US irradiation. DCFH-DA (10 μM) was employed as the intracellular ROS sensor. Scale bar: 25 μm. f CRT exposure and HMGB1 release in 4T1 cells treated with different formulations, following by CLSM. Scale bar: 25 μm. g Flow cytometry analysis of CRT exposure in different groups. h HMGB1 release in cell supernatant after various treatments. i ATP secretion in different groups. Data are expressed as means ± SD (n = 3). Statistical significances were calculated via one-way ANOVA Tukey's multiple comparisons test, *P < 0.05, **P < 0.01, ***P < 0.001

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Journal of Nanobiotechnology

ISSN: 1477-3155

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