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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: 3D printed hydrogel scaffolds combining glutathione depletion-induced ferroptosis and photothermia-augmented chemodynamic therapy for efficiently inhibiting postoperative tumor recurrence

Fig. 6

In vitro anticancer efficacy of Gel-SA-CuO hydrogel scaffolds. Viability of H22 cells treated with indicated scaffolds with (a) or without (b) NIR irradiation. c Effect of incubation time on the viability of H22 cells treated with indicated scaffolds. d Fluorescence images of H22 cells after various treatments and stained with calcein-AM and PI. Scale bars represent 200 μm. e Detection of intracellular ROS generation of H22 cells after various treatments and stained with DCFH-DA. Scale bars represent 100 μm. f Measurement of GSH/GSSG ratio in H22 cells with indicated treatments. g GPx activity of H22 cells with various treatments. h The GPX4 expression of H22 cells with various treatments. i Detection of cell apoptosis of H22 cells under indicated treatments

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