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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin

Fig. 2

DHA@MIL-101 NRs treated Lewis cells exhibited enhanced cytotoxicity boosted by ROS generation and ferroptosis. A Schematic illustration of DHA@MIL-101 NRs mediated CDT and ferroptosis. B Different concentrations of DHA, MIL-101 NRs, and DHA@MIL-101 NRs were used to treat Lewis cells. Cell viability was analyzed by CCK-8. C, D Lewis cells were incubated with the DHA, MIL-101 NRs and DHA@MIL-101 NRs for 12 h and intracellular iron ions were detected using a PGSK probe and flow cytometry. E–F Lewis cells were incubated with DHA@MIL-101 NRs at various times to screen for the optimal time for inducing the peak of ROS. Cellular ROS generation was measured by DCFH-DA staining and flow cytometry. H2O2 treatment was a positive control. G, H) Lewis cells were incubated with DHA, MIL-101 and DHA@MIL-101 NRs for 18 h. Intracellular ROS was detected by DCFH-DA staining. I GSH content in Lewis cells was measured by a GSH kit. J, K, M Lipid peroxidation was detected using a C11-BODIPY probe with flow cytometry and confocal microscopy. L The expression of GPX4 and COX-2 in Lewis cells was measured by WB. N The MDA concentration in LLC was measured. All values are means ± SD (n = 3, * p < 0.05)

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