Fig. 5

DHA@MIL-101 NRs exhibited significant efficacy in vivo and induced powerful apoptosis, ferroptosis, and DNA damage in tumor grafts. Caudal vein injection of DHA, MIL-101 NRs and DHA@MIL-101 NRs in 200 μL of PBS per mouse. A The mice were sacrificed and tumor grafts were harvested, then photographed. B The weight of tumor grafts was measured. C The tumor growth of Lewis cells-bearing mice was monitored during the whole experiment. D The tumor tissues were prepared for paraffin sections, then labeled with DAPI, Tunel and PI. The apoptosis and necrosis were assayed using confocal microscopy. E, F Expression of GPX4 (biomarkers of ferroptosis) and p53 (biomarkers of DNA damage) was detected with IHC staining. Values are means ± SD (n = 5, * p < 0.05)