Fig. 7

PCM-MSN@LA reduced cardiomyocyte apoptosis. a The viability of cardiomyocytes isolated from each group with different treatments (n = 6). Data were presented as mean ± SD. *P < 0.05; **P < 0.01. b Photomicrographs of cardiomyocyte apoptosis by TUNEL staining (scale bar = 120 μm) and ROS burden by DCFH-DA staining (scale bar = 200 μm) after individualized treatments. DAPI (blue; nucleic); TUNEL positive (red; apoptotic nucleic; white arrow); ROS positive (green). c Quantitative analysis of TUNEL positive cells which were representative of apoptosis (n = 6). Data were presented as mean ± SD. *P < 0.05; **P < 0.01. d Quantitative analysis of intensity of ROS fluorescence treatments (n = 6). Data were presented as mean ± SD. *P < 0.05; **P < 0.01