Fig. 3

NIR laser irradiation of pTRG-treated tumors induced the activation of conventional dendritic cells (cDCs) in tumor-draining lymph nodes (tdLNs). A Schematic illustration of the analysis method for DC activation in tdLNs. B cDC subset analysis in tdLNs via flow cytometry. C The mean fluorescence intensities (MFIs) of CD40, CD80, CD86, MHC I, and MHC II in cDC1 (upper panel) and cDC2 (lower panel) were measured via flow cytometry (n = 6, two-way ANOVA, mean ± SEM, *p < 0.05, **p < 0.01). D Serum concentrations of indicated cytokines were analyzed using enzyme-linked immunosorbent assays (ELISAs) (n = 6, two-way ANOVA, mean ± SEM, **p < 0.01)