Fig. 6
pTRG and NIR laser irradiation prevented 4T1 tumor growth. BALB/c mice were injected s.c. with 1 × 106 4T1-iRFP cells. On the 7th day after tumor implantation, the mice were i.t. treated with PBS, poly I:C, Gel, pGel, TRG, and pTRG and then subjected to NIR laser irradiation (1.5 W/cm2, 5 min). A iRFP imaging in 4T1 tumor-bearing mice on day 20 (n = 6). B Tumor growth curves of 4T1 mice (n = 6, significance was determined by the log-rank test, mean ± SEM **p < 0.01). C–F The surviving mice from the first 4T1 challenge were subjected to a second challenge with 4T1 cells (0.5 × 106) by i.v. injection on the 30th day following the first tumor injection. C The mouse survival rates were monitored (n = 5 per group). D iRFP fluorescence images and E photographs of metastatic lung cancer on the 10th day after the second 4T1 challenge (n = 6). F Representative images of hematoxylin and eosin (H&E) staining of the lung tissues (n = 6)