Skip to main content
Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Biomimetic and temporal-controlled nanocarriers with ileum transporter targeting for achieving oral administration of chemotherapeutic drugs

Fig. 5

Cellular trafficking pathway of GCA-NPs. A The emission spectrum of basolateral medium was assayed after incubated with DiO/DiI@GCA-NPs for 30 min, 60 min, 120 min, 180 min. B FRET efficiency of DiO/DiI GCA-NPs and DiO@GCA-NPs + DiI@GCA-NPs after transport across Caco-2 cell monolayers for 30 min, 60 min, 120 min, 180 min. The excitation wavelength was 480 nm. C Colocalization of Cou-6@GCA-NPs and Golgi apparatus (GA). Cell nucleus was stained with DAPI (blue) and GA complex was labeled with GA tracker (red). (scale bar: 25 μm, magnification: 630 ×). D Colocalization analysis of Cou-6@GCA-NPs and GA. E Colocalization of Cou-6@GCA-NPs and endoplasmic reticulum (ER). Cell nucleus was stained with DAPI (blue) and ER were labeled with ER tracker (red). (scale bar: 10 μm, magnification: 630 ×). F Colocalization analysis of Cou-6@GCA-NPs and ER. G Cellular colocalization of Cou-6@GCA-NPs and Cou-6@QL with lysosome at 60 min and 180 min, respectively. Cell nucleus was labeled with DAPI (blue) and lysosomes were stained with lysotracker (red). (scale bar: 10 μm, magnification: 630 ×). Data is presented as mean ± SEM, n = 3, ***P < 0.001

Back to article page