Fig. 3

In vitro targeting of iRGD-Exos. A Confocal microscopy images of 8505C cells incubated with PKH26-blank-Exos and PKH26-iRGD-Exos at 4 h. Nuclei were stained with DAPI (blue). Fluorescence from PKH26 (red) and DAPI (blue) was observed. The scale bar is 10 μm. B Flow cytometric analysis of PKH26-iRGD-Exos binding to 8505C cells. Exosomes were labeled with PKH26 and incubated with 8505C for different lengths of time (3 h, or 6 h). ***P < 0.001