Fig. 2

Intracellular uptake and in vitro anticancer efficacy of Abplatin^(IV). A Intracellular uptake of Abplatin^(IV)@Cy5.5 by BEL-7404 cells via confocal laser scanning microscope (CLSM), scale bar = 40 µm. B Semi-quantitative study of the intracellular uptake of Abplatin^(IV)@Cy5.5 via flow cytometry. C Intracellular Pt uptake of the cells treated with various drugs (10 μM Pt) for 1, 4, and 7 h respectively via ICP-MS. D Pt-DNA adducts of cells treated with cisplatin, CisPt(IV), and Abplatin^(IV) via ICP-MS. E Fluorescence distribution of Abplatin^(IV)@Cy5.5 at 12 h in different focal planes of 3D tumor sphere, scale bar = 100 µm. F In vitro anticancer activity of cisplatin, CisPt(IV) and Abplatin^(IV). G Cell apoptosis in 3D tumor sphere induced by various drugs (10 µM) in 3D tumor spheres at 24 h, scale bar = 200 µm