Fig. 6
From: Dual-binding nanoparticles improve the killing effect of T cells on solid tumor
The DBMN helped T cells adhere to tumor cells. A The CD8 + T cell–Nanoparticle–Tumor cell bridge was built when DBMN-T were cultured with 4T1 tumor cells. Red, DiD-labeled 4T1 cell membrane; Green, FITC-DBMN; Blue, Hoechst labeled CD8 + T cell Nucleus. Scale bar, 10 μm. B Pattern diagram of adhesion test. Circulating T cells attracted under a magnetic field and the number of CD8 + T cells (C) adhered to 4T1 cells counted by Image J, n = 4. D Pattern diagram of rinsing test. Free medium rinsed CD8 + T cells attached on 4T1 cells and the number of remaining CD8 + T cells (E) counted by Image J, n = 4. F Fluorescence images of normal T cells, SBMN-T and DBMN-T, respectively. Left column, under magnetic field for 48 h; Right column, without magnetic field for 24 h after previous step. Statistical analyses were carried out by Prism graphpad 8.0. All error bars are expressed as ± SD. Comparisons between two or several groups were analyzed using unpaired Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001