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Table 2 Average results of SERS qRT–PCR results for real samples

From: A pump-free and high-throughput microfluidic chip for highly sensitive SERS assay of gastric cancer-related circulating tumor DNA via a cascade signal amplification strategy

Day

SERS (fM)

qRT–PCR (fM)

Relative error (%)

PIK3CA E542K

TP53

PIK3CA E542K

TP53

PIK3CA E542K

TP53

1

0.214

0.198

0.204

0.188

4.67

5.05

5

0.333

0.304

0.317

0.289

4.80

4.93

9

0.466

0.396

0.443

0.376

4.94

5.05

13

0.669

0.542

0.637

0.514

4.78

5.17

17

0.891

0.839

0.843

0.797

5.38

5.01

21

1.143

1.081

1.084

1.017

5.16

5.92

25

1.556

1.455

1.476

1.377

5.14

5.36

29

2.131

1.983

2.035

1.889

4.50

4.74