Fig. 1
From: Biomimetic nanobubbles for triple-negative breast cancer targeted ultrasound molecular imaging
In vitro characterization of CCM-based NBs. a Western blot of different cellular fractions from MDA-MB-231 cells isolated by a differential centrifugation method for various cellular markers. The samples were probed using antibodies against GAPDH, Histone 3, Cytochrome C, and N-Cadherin. b Representative digital photographs of NBs before and after microfluidizer processing; c Particle zeta potential and size distribution measured using DLS; d relative concentration of NBs using single particle optical sensing. e Composition of each bubble tested in this study; (+) and (−) indicate the presence and absence of surfactant respectively in the formulation. f Characteristic summary of TNBC targeted NB75%PL25%CCM (NBCCM) and non-targeted NBs (NBctrl). WCL: whole cell lysate fraction; CY: cytoplasm fraction; MI: mitochondria fraction; NU: nucleus fraction; Data are shown as Mean ± SD. *P < 0.05,**P < 0.01, ****P < 0.0001