Fig. 5

The mechanisms of antioxidant, anti-inflammatory and anti-apoptotic effects of ATX-PPS-NP.A Confocal images of intracellular ROS level stained by DCFHDA (green fluorescence) in ATX (1 μg/ml), PPS-NP (drug free) and ATX-PPS-NP (1 μg/ml) pretreated HEI-OC1 cells, followed by CDDP (60 μM, 24 h) administration. B Quantifications of MFI of DCFHDA. C GSH levels. D IL-6 levels. E–F The expression levels and qualifications of apoptosis-associated proteins evaluated by Western blot analyses. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001 vs control, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs CDDP, ^&&& p < 0.001 vs ATX-PPS-NP