Fig. 4

The effect of LANP-siSRI and LANP-PTX-siSRI in vitro. a–b Expression of SRI and ABCB1 mRNA in A2780/PTX and A549/PTX cells after the knockdown of SRI by LANP-siSRI determined by qRT-PCR. c–d Expression of SRI and ABCB1 protein in A2780/PTX and A549/PTX cells after the knockdown of SRI by LANP-siSRI detected by Western blot. e Measurement of calcium ion concentration in A2780/PTX and A549/PTX cells after the knockdown of SRI by LANP-siSRI detected by flow cytometry. f–g Flow cytometry data analysis of fluorescence intensity reflected calcium ion concentration in A2780/PTX (f) and A549/PTX (g) after the knockdown of SRI by LANP-siSRI. h Heatmap revealing the overexpression of S100 series genes in A2780/PTX detected by mRNA sequencing. i-j Expression of S100 series genes in A2780/PTX (i) and A549/PTX (j) detected by qRT-PCR. k–l Cell viability detection in A2780/PTX (k) and A549/PTX (l) after the knockdown of SRI by LANP-siSRI. m–n Cell viability detection in A2780/PTX (m) and A549/PTX (n) after treatment of LANP-PTX-siSRI. *P < 0.05; **P < 0.01