Fig. 3
From: Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells
Immunofluorescence staining of osteopontin in MSCs cultured on the NW scaffold. MSCs were cultured for a 2 days, b 1 week or c 2 weeks and then stained for F-actin (green) and OPN osteogenic marker (red). Representative images of n = 2 independent experiments. d Box plots showing the corrected total cell fluorescence quantification of OPN following the culture of MSCs on the NW surface after 1 and 2 weeks. n = 28 imaged cells, *p < 0.01. NC negative control (cells cultured on a regular tissue culture-treated plastic).