Fig. 4
From: Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells
Osteopontin staining in MSCs cultured on NWs exposed to a magnetic field for various durations of time. MSCs were cultured for a 2 days, b 1 week, or c 2 weeks, and a magnetic field with an intensity of 250 mT and a frequency of 0.1 Hz was applied 12 h per day (i.e., 12 h with magnetic field and 12 h without magnetic field). The cells were then stained for F-actin (green) and OPN (red). NC negative control. d Extracellular OPN expression after 1 week (left) and 2 weeks (right) exposure to a magnetic field under the previously mentioned parameters. Representative images of n = 2 independent experiments.