Fig. 3

Effects of t-BOOH on cell viability, apoptosis and cell cycle of PC12 cells reversed by TSIIA@SeNPs-APS. A The viability of PC12 cells under different concentrations of TSIIA@SeNPs-APS for 24 h (calculated by Se concentration). B The viability of PC12 cells pretreated with t-BOOH and then treated with SeNPs, SeNPs-APS and TSIIA@SeNPs-APS for 24 h, respectively, at the concentration of 1.25 and 2.5 μM (calculated by Se concentration). Significant difference between the 2.5 μM TSIIA@SeNPs-APS group and the other groups is indicated by *P < 0.05, **P < 0.01, ***P < 0.001. C The viability of PC12 cells pretreated with t-BOOH and then treated with APS and TSIIA@SeNPs-APS for 24 h, respectively, at the concentration of 0.32 and 0.64 μg/ml (calculated by APS concentration). **P < 0.01. D The viability of PC12 cells pretreated with 100 μM t-BOOH and then treated with TSIIA and TSIIA@SeNPs-APS for 24 h, respectively, at the concentration of 0.22 and 0.45 ng/ml (calculated by TSIIA concentration). *P < 0.05, **P < 0.01. E Flow cytometry analysis of the effects of SeNPs, SeNPs-APS and TSIIA@SeNPs-APS on the cell cycle distribution of PC12 cells pretreated with t-BOOH. F Proportion of the cell cycle in (E). G Western blot analysis of CDK2. Lane 1–5: groups of control, t-BOOH, t-BOOH + SeNPs, t-BOOH + SeNPs-APS and t-BOOH + TSIIA@SeNPs-APS, respectively. H Annexin V-FITC/PI double-staining to evaluate the effects of SeNPs, SeNPs-APS and TSIIA@SeNPs-APS on the apoptosis of PC12 cells pretreated with t-BOOH. I Quantitative analysis of the proportion of apoptosis in (H). J Western blot analysis of pro-Caspase-3 and pro-Caspase-9. Lane 1–5: the same as groups in G