Fig. 3

Immuno-activation in vitro. A CRT exposure in C1498 cells with different treatments analyzed by flow cytometry. B High-mobility group protein B1 (HMGB1) detected by enzyme-linked immunosorbent assay (ELISA) (n = 3). C Adenosine triphosphate (ATP) detected by the enhanced ATP assay kit (n = 3). D Statistical analysis of (E). E DC maturation (CD80⁺CD86⁺ of CD11c⁺ DCs) after cocultured with C1498 cells with different treatments. Data are shown as mean ± SD. P values were calculated by Student’s t-test, *p < 0.05, **p < 0.01