Fig. 5

Immuno-activation in vivo. A DC maturation (CD80⁺CD86⁺ of CD11c⁺ BMDCs) in the lymph nodes detected by flow cytometry. B Statistical analysis of (A) (n = 3). C Proportion of peripheral CD3⁺ T cells on day 8. D Statistical analysis of (C) (n = 3). E Proportion of CD8⁺ T cells and CD4⁺ T cells (gated on CD3⁺ T cells) on day 8. F Statistical analysis of proportion of peripheral CD8⁺ T cells (n = 3). G Proportion of CD3⁺ T cells in bone marrow. H CD8⁺ T cells and CD4⁺ T cells (gated on CD3⁺ T cells) on day 22. I Statistical analysis of (G) (n = 3). J Statistical analysis of proportion of CD8⁺ T cells in bone marrow (n = 3). K TNF-α and (L) IFN-γ in serum on day 8 detected by ELISA (n = 3). Data are shown as mean ± SD. P values were calculated by Student’s t-test, *p < 0.05, **p < 0.01, ns means no significant difference