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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Insulin-incubated palladium clusters promote recovery after brain injury

Fig. 2

Characterizations and multiple ROS scavenging ability of Pd@insulin clusters. A Scheme illustration of Pd@insulin synthesis via the protein biomimetic method. B HRTEM images of Pd@insulin (Scale bar: 10 nm left, 1 nm right). C Superoxide anion E hydroxyl radical, and G H2O2 scavenging ability of Pd@insulin (n = 4). Time depended D superoxide anion and F hydroxyl radical scavenging ability of Pd@insulin by EPR spectrum. Comparison of H superoxide anion I hydroxyl radical J H2O2 scavenging ability between Pd@insulin cluster, insulin, and Pd2+ (n = 5). K Adsorption energies profiles of catalytic process of the SOD and CAT processes for Pd, and geometry structures of the intermediate states (red and white dots represent oxygen and hydrogen atoms respectively; * represents absorption state). M Intracellular ROS scavenging ability evaluation via N2a, BV2, and A172 cells (Rosup is positive control, green fluorescence represents ROS positive) (Scale bar 200 μm), and N corresponded quantitative results (n = 3). Data are all shown as mean ± SD. Statistical analysis of (C, E, G, H, I, J, and N) was performed by one-way ANOVA with a Tukey post hoc test

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