Fig. 4
From: Insulin-incubated palladium clusters promote recovery after brain injury
Pd@insulin clusters cross the BBB and aggregate in the injured cortex after TBI. A In vivo blood pharmacokinetic curve. B Excretive Pd2+ concentration in mice urine and feces (n = 3). C In vivo fluorescence images of control mouse (left), and mouse treated with Pd@insulin (middle) or insulin (right), respectively. D Biodistribution of Pd@insulin in major tissues of mice at 15 min post treatment. E Fluorescence images of brain tissue post insulin or Pd@insulin administration. F Pd2+ concentration in normal mice brain (after perfusion). Immunofluorescent staining of TBI mice for G different brain area including cortex, hippocampus and thalamus. H Immunofluorescent staining of TBI mice cortex (MAP2, Iba1 and GFAP on behalf of neuron, microglia and astrocyte, respectively) (Scale bar 100 μm). Data are all shown as mean ± SD.