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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Small extracellular vesicles derived from dermal fibroblasts promote fibroblast activity and skin development through carrying miR-218 and ITGBL1

Fig. 1

Characterization of skin thickness and SEVs from DFs. A Skin thickness (left), Sirius red (middle), and HE stain (right) of CH and LW pig skin tissue. Scare bars of Sirius/HE = 1000/100 µm. B Transmission electron microscope images of SEVs derived from DFs of CH and LW pigs. Scare bars = 100 nm. C Size distribution of CH/LW-SEVs by nanoparticle tracking analysis. n = 3. D Western blot bands of CD9, CD63, and CD81 in secreting cells and CH/LW-SEVs. n = 3. E CD9 and CD63 of CH/LW-SEVs were detected by flow cytometry. n = 3. F Images of location between CH-SEVs and CHDFs by confocal microscopy. Cells (105) were incubated with 10 µg SEVs labelled with PKH67 (green) for 24 h. The supematant of free SEVs-labelled was used as a negative control (NC). Scare bars = 20 μm. CH, Chenghua; LW, Large White; HE, hematoxylin-eosin. The data was calculated using student’s t test. Data are expressed as means ± SEM

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