Fig. 3

Bioinformatics analysis for differentially expressed miRNAs between CH-SEVs and LW-SEVs. A Heatmap of differential miRNAs between CH-SEVs and LW-SEVs. n = 3. Pseudo-colors show expression levels from red (high) to blue (low). B Volcano plots of differential miRNAs between CH-SEVs and LW-SEVs. Red and blue plots represent up and down-regulated miRNAs, respectively. n = 3. C KEGG for target genes from differential miRNAs. D GO for these target genes from differential miRNAs. E Expression levels of miR-218 in CH/LW-SEVs, CHDFs, and LWDFs. n = 3. The data was calculated using student’s t test and two-way ANOVA followed by Bonferroni’s multiple comparisons test. Data are expressed as means ± SEM; with P* < 0.05, P** < 0.005, P*** < 0.001