Fig. 4

Effect of miR-218 on fibroblast and skin tissue. Expression levels of COL1A1 mRNA (A) and collagen I content (B) in CHDFs transfected with miR-218. n = 3. C Proliferation of CHDFs transfected with miR-218. n = 3. Scare bar = 100 μm. D Cell cycle of CHDFs transfected with miR-218. Data are the percentage of G1, G2/M and S. n = 3. E Wound recovery area of CHDFs treated with miR-218. Objective area is between the two red lines. n = 3. F Images of collagen gel contraction in CHDFs treated with miR-218. n = 3. Skin thickness (G) and collagen I content (H) of mice injected miR-218 agomir. n = 3. The data was calculated using student’s t test and two-way ANOVA followed by Bonferroni’s multiple comparisons test. Data are expressed as means ± SEM; with P* < 0.05, P** < 0.005, P*** < 0.001