Fig. 5

miR-218 targeted TGIF2 and TGIF2 repressed fibroblast activity via TGFβ1-SMAD2/3 pathway. A Sequence alignment between TGIF2 and miR-218. B Relative luciferase activity of between TGIF2 and miR-218. n = 3. C Expression levels of COL1A1 mRNA in CHDFs transfected with TGIF2. n = 3. D Proliferation of CHDFs transfected with TGIF2. n = 3. Scare bar = 100 μm. E Cell cycle of CHDFs transfected with TGIF2. Data are the percentage of G1, G2/M and S. n = 3. F Wound recovery area of CHDFs treated with miR-218 and TGIF2. Objective area is between the two red lines. n = 3. G Cell cycle of CHDFs co-treated with miR-218 and TGIF2. Data are the percentage of G1, G2/M and S. n = 3. H Protein levels of the related genes in CHDFs. n = 3. The data was calculated using student’s t test and two-way ANOVA followed by Bonferroni’s multiple comparisons test. Data are expressed as means ± SEM; with P* < 0.05, P** < 0.005, P*** < 0.001