Fig. 4

Example of p-score calculation (In this case based on images of platelets with VEGF staining). A set of 10 platelets from a certain (co-)culturing category are randomly selected (in this example from resting (R) platelets). For each of the 5 imaging parameters, the values determined for the 10 platelets are compared against the corresponding probability curves, in this example for VEGF, and for all the different platelet categories (A–E). The probabilities of the 10 platelets to belong to a certain category are then added for each parameter (A–E insets). F The combined p-score calculated for each co-culturing condition is obtained by adding the sum probabilities for all parameters for the given culturing category. The set of 10 platelets are finally classified into the category with the highest combined p-score, in this example into R