Fig. 3

RCM-fiber-BMMSCs remodel collagen deposition at the wound areas. A Masson’s trichrome staining for tissue sections (left) with high-resolution images of collagen deposition in the epidermis, dermis and hypodermis (right) in diabetic mouse wounds. Scale bar = 1 mm (left) or 50 μm (right). B Picrosirius red staining for the collagens in the wound regions of diabetic mice after the treatment at day 15. Scale bar = 50 μm. C Comparisons of Collagen 1 and Collagen 3 percentages in the wound areas among five groups. D Immunostaining for 8-OHdG (i), CK10 (ii) and CD31 (iii) in the wound areas upon different treatments. Scale bar = 50 μm. Data were represented as mean ± SD. Differences were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. (mice: n ≥ 8) NS: non-significant, *P < 0.05, **P < 0.01