Fig. 4

RCM-fiber-BMMSCs treatment leads to decreased inflammation in diabetic wound regions. A Immunofluorescence staining for F4/80 (red), CD206 (green), CD86 (green) and TNF-α (green) in the wound tissues. Scale bar = 50 μm. B Comparisons of the proportions of F4/80⁺CD86⁺, F4/80⁺TNF-α⁺ and F4/80⁺CD206⁺ cells in the wound areas among different groups. C, D Expression levels of IL-1β (C) and IL-10 (D) in diabetic cutaneous wound areas on day 7 by real-time PCR. E Relative expression levels of Ccl2, Lif, TNFAIP6 and Lcn2 in the diabetic skin wounds treated with different nanofibers. Data were represented as mean ± SD. Differences were analyzed by using one-way ANOVA followed by Tukey’s multiple comparison test. (mice: n ≥ 8) NS: non-significant, *P < 0.05, **P < 0.01