Fig. 7
Physiochemical and biological characterization of the TCM-fibers. A Representative fluorescent images of the TCM-fibers after coumarin (for PLGA nanofiber) and Dil (for lipid bilayer) staining. Scale bar = 20 μm. (B Detection of Na+/K+ ATPase and CD11c proteins on the TCM-fibers and Un-fibers (without cell membrane) by Western blotting. C Representative images of TCM-fibers morphology by a scanning electron microscopy. Scale bar = 10 μm (left) or 5 μm (right). D Size distribution of the TCM-fibers. E Fluorescent images of human BMMSCs (hBMMSCs) cultured on the Un-fibers or RCM-fibers by CFDA-SE staining. Scale bar = 5 μm. F CCK-8 assays for cell proliferation of hBMMSCs at day 1, day 3, day 5 and day 7, respectively. G Representative images of the wound areas covered by HaCaT cells at 12, 24 and 36 h in cell migration assay exposed to H2O2 in vitro. Scale bar = 25 μm. H Comparisons of the percentages of the residual wound areas covered by HaCaT cells at different time points normalized to the original would areas. Data were represented as mean ± SD. Differences were analyzed by one-way ANOVA with Tukey’s multiple comparison tests.(n ≥ 3) *P < 0.05, **P < 0.01