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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration

Fig. 5

miR-762 and iPSCs-KCs-Exos promote migration of keratinocyte and endothelial cells by targeting PML. A Predicted miR-762 target sequences in PML 3’-UTR and the mutated nucleotides in the 3′UTR of PML. B The relative luciferase activity in 293 T cells with psiCHECK-PML-wt-3′UTR (WT) or psiCHECK-PML-mut-3’UTR (MUT) and miR-762 mimics co-transfection was evaluated by luciferase reporter assay. C, D qRT–PCR and Western blot analysis of PML expression in HaCaT cells and HUVECs which were treated with control, miR-762 mimics and iPSCs-KCs-Exos (Exos). E Representative images of the wound healing assay and quantitative analysis of the wound healing rates of HaCaT cells, which were treated with a control, b miR-762 mimics, c iPSCs-KCs-Exos (Exos), d pcDNA3.1-PML (PML), e miR-762 mimic plus pcDNA3.1-PML (PML), f iPSCs-KCs-Exos (Exos) plus pcDNA3.1-PML (PML). Scale bar, 200 μm. F Images of migrated HUVECs and quantitative analysis of the migrated cells, which were treated with a control, b miR-762 mimics, c iPSCs-KCs-Exos (Exos), d pcDNA3.1-PML (PML), e miR-762 mimic plus pcDNA3.1-PML (PML), f iPSCs-KCs-Exos (Exos) plus pcDNA3.1-PML (PML). Scale bar, 200 μm. *P < 0.05, **P < 0.01

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