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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration

Fig. 6

PML-regulated ITGB1 is involved in the promotion of keratinocytes and endothelial cell migration by iPSCs-KCs-Exos. A, B qRT–PCR and Western blot analysis of ITGB1 expression in HaCaT cells and HUVECs which were treated with control siRNA or PML siRNA. B Western blot analysis of protein expression of ITGB1 in each group. C, D qRT–PCR and Western blot analysis of ITGB1expression in HaCaT cells and HUVECs which were treated with control, miR-762 mimics, iPSCs-KCs-Exos. E Representative images of the wound healing assay and quantitative analysis of the wound healing rates of HaCaT cells, which were treated with a control, b miR-762 mimics, c iPSCs-KCs-Exos (Exos), d pcDNA3.1-ITGB1 (ITGB1), e miR-762 mimic plus pcDNA3.1- ITGB1 (ITGB1), f iPSCs-KCs-Exos (Exos) plus pcDNA3.1- ITGB1 (ITGB1). Scale bar, 200 μm. F Images of migrated HUVECs and quantitative analysis of the migrated cells, which were treated with a control, b miR-762 mimics, c iPSCs-KCs-Exos (Exos), d pcDNA3.1-ITGB1 (ITGB1), e miR-762 mimic plus pcDNA3.1- ITGB1 (ITGB1), f iPSCs-KCs-Exos (Exos) plus pcDNA3.1- ITGB1 (ITGB1). Scale bar, 200 μm. *P < 0.05, **P < 0.01

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