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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Differentiated kidney tubular cell-derived extracellular vesicles enhance maturation of tubuloids

Fig. 2

EV-OAT1 promote changes in epithelial transporters and transcription factors genes in tubuloids. a Conditioned medium and extracellular vesicles from ciPTEC-OAT1 (CM-OAT1 and EV-OAT1) upregulate OAT1 in tubuloids (TUB). The graph shows the changes in the mRNA levels in the tubuloids in different experimental conditions indicated in the abscissa (CTR represents standard differentiation protocol; EV-14.4 indicates EVs derived from ciPTEC 14.4; EV dep indicated CM-OAT1 depleted of EVs; Medium indicates the differentiation medium submitted to the same process of concentration for EVs and that was incubated with the tubuloids). b EV-OAT1 promote OAT1 upregulation in the tubuloids in a dose-dependent manner. The graph indicates the mRNA OAT1 levels stimulates with EV-OAT1 with a single dose (TUB EV-OAT1 1st) or three doses in a single stimulation (TUB EV-OAT1 3st). c The graph shows the changes in OAT1 mRNA levels in the tubuloids after different stimulation timepoints. The abscissa indicates the condition: TUB EV-OAT1 indicates normal stimulation protocol with administration of 3 doses in 3 different days within 7 days; 3st* indicates the stimulation of 3 doses in a singles stimulus in the beginning of differentiation protocol (day 1), while 3st** indicates the same single stimulus at the end of the protocol (day 5). d CM-OAT1 and EV-OAT1 promote the upregulation of other epithelial transporters in tubuloids. The graph shows the mRNA levels of epithelial transporters (ATP1, MRP3, MRP2, BCRP and OAT3) of tubuloids cultured in the different experimental conditions (abscissa). e Transcription factors associated with drug transport are upregulated by CM-OAT1 and EV-OAT1. The graph shows the mRNA levels of transcription factors in the tubuloids (HNF1A, HNF4A, HNF1B). In all graphs, the data is expressed in relative quantification (RQ) with respect to the control condition (TUB CTR) (n = 5). Data represent mean ± SEM, *p < 0.05 with respect to TUB CTR group and **p < 0.05 with respect to TUB EV-OAT1 3st* group

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