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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Differentiated kidney tubular cell-derived extracellular vesicles enhance maturation of tubuloids

Fig. 6

Tubuloid-derived cell form tight monolayer on microfluidic hollow fiber-based platform and CM-OAT1 and EV-OAT1 improve cilia density. a Scheme of the microfluidic hollow fiber-based platform and how tubuloid-derived cells are organized, and selectively transport molecules from one compartment to another. b Representative confocal image of tubuloids-derived cells cultured on hollow fibers in the presence of EV-OAT1. The ZO-1 immunostaining (in green) shows the presence of a tight epithelial monolayer on the fiber. c Representative y–z confocal image of the curved surface of the fiber and the polarized tubuloid-derived cells cultured with EV-OAT1. The acetyl-α-tubulin immunostaining in the apical region (in green) indicates the cilia structure formation and Na+/K+-ATPase is localized to the basolateral region (in red). d Higher magnification of tubuloids cells on the fibers with α-tubulin (in green) and Na+/K+-ATPase (in red) staining. e The cilia structures (in green) are mainly observed when the apical region is in focus, while in f the presence of Na+/K+-ATPase (in red) is mainly observed in the basolateral region. In all images, scale bar = 50 µm. g Quantification of total cilia perimeter present in the tubuloids-derived cells under the different experimental conditions. The graph shows the total cilia perimeter with respect to the total number of cells. Data represent mean ± SEM, *p < 0.05 compared to TUB CTR group

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