Fig. 6
Micro-CT measurements and histological examination of calvarial defects. A Implantation of osteoblasts encapsulated inside Alg-nHA-Col microspheres cross-linked with Sr2+. B, C CBCT and micro-CT scanning reveal de novo bone formation in the calvarial defect in the rat model to assess the osteogenic capacity of osteoblasts encapsulated inside Alg-nHA-Col microspheres cross-linked with Sr2+ after 8 weeks. Regenerated bone is pseudo-colored red. D Quantitative analysis of bone formation based on micro-CT images. Significance is indicated (p < 0.05). n = 4 for each group. E Microanatomical demonstration of repair of critical-size defects in the rat calvaria after 8 weeks of transplantation stained with H & E