Fig. 5

Overexpression of Cxcr3 in MSCs. a Representative bright field (BF) and green fluorescence images of MSC^eGFP and MSC^Cxcr3. MSCs were transduced with lentivirus to express eGFP alone (MSC^eGFP) or in combination with Cxcr3 (MSC^Cxcr3). b Analysis of Cxcr3 mRNA expression in MSC^eGFP and MSC^Cxcr3.c Western blot of Cxcr3 in total cell lysates of MSC^eGFP and MSC^Cxcr3. CypB, cyclophilin B, used as housekeeping gene. d Cxcr3 overexpression promoted the in vitro migration of MSCs toward Cxcl10. Representative images and quantification analysis of transwell migration assay were shown. e Quantification analysis of transwell migration assay of MSC^Cxcr3 after incubating with vehicle (PBS) or TAT-CPNPs (NPs). Scale bars, 100 µm. *P < 0.05, ***P < 0.001; error bars, SEM