Skip to main content
Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Unveiling the improved targeting migration of mesenchymal stem cells with CXC chemokine receptor 3-modification using intravital NIR-II photoacoustic imaging

Fig. 7

MSCCxcr3 exhibited enhanced migration toward inflamed ears in vivo. a Representative PA MAP images showing more significant accumulation of TAT-CPNPs labeled MSCCxcr3 than MSCeGFP in the inflamed ears of CHS mice over time. PA images were captured at 15 min, 3 h or 7 h after mice receiving labeled MSCeGFP or MSCCxcr3 injections, respectively. The imaging procedure for each group was repeated on 3 mice and complemented in Fig. S4. White boxes correspond to magnified region in upper left. White dashed circles indicate TAT-CPNPs labeled MSCeGFP or MSCCxcr3. Scale bar, 0.5 mm. b Normalized area of total labeled MSCeGFP or MSCCxcr3 in PA images captured 15 min, 3 h and 7 h after labeled MSCeGFP or MSCCxcr3 injection (n = 3 per group). c Normalized area of extravascular labeled MSCeGFP or MSCCxcr3 in PA images captured 15 min, 3 h and 7 h after labeled MSCeGFP or MSCCxcr3 injection (n = 3 per group). d Normalized area of blood vessels of inflamed ears of CHS mice injected with MSCeGFP or MSCCxcr3 (n = 3 per group). e Histological examination verified the significantly increased presence of labeled MSCCxcr3 compared with labeled MSCeGFP in the inflamed ear of CHS mice by immunostaining of GFP. The red arrows in the magnified images denoted GFP-expressing MSCs. Scale bars, 25 µm. ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001; error bars, SEM

Back to article page