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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: A universal strategy for the fabrication of single-photon and multiphoton NIR nanoparticles by loading organic dyes into water-soluble polymer nanosponges

Fig. 4

a Biocompatibility of PSMA NPs loaded with different dyes. Fluorescent images of cells labeled with b PSMA-242, YI-1, ADF1 single staining or c PSMA-(ADF1 + ADF2) double staining and tracked by fluorescence microscopy. Scale bars = 20 μm. Multiphoton imaging of d PSMA-242 and e PSMA-YI-1 excited at 1080 nm. In vivo two-photon fluorescence (red color) and second harmonic generation (green color) imaging of dye-loaded PSMA NPs circulated in the blood vessels of a mouse ear. f PSMA-242; g PSMA-YI-1; excitation wavelength: 1080 nm. Fields of view: 317 × 317 μm

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