Fig.1

Changes of intracellular protein nanoparticle-related osmotic pressure, Vimentin tension in response to AngII or BK and treatment with cytoskeleton stabilizers. A Cytoplasm osmotic pressure of A549 cells were measured using a freezing point osmometer in response to AngII (10 μM) or BK (10 μM) stimuli vs time. B Cytoplasm osmotic pressure of A549 cells in response to AngII or BK, and treatment with jasplakinolide (JK, 1 μM), taxol (TAX, 10 μM), or both agents. C The count rate of PN in A549 cells. D–E Nano size distribution of protein granules in the cytoplasm. F Control, AngII-, and BK-treated A549 cell monolayers were stained for Phalloidin (FITC) and α-tubulin (TRITC). Images were generated from confocal laser microscopy after immunofluorescence staining. Scale bar:10 μm. G Normalized CFP/FRET ratios in Vimentin tension under the different treatments. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001