Fig.2

Effects of ASC siRNA on intracellular protein nanoparticle-related osmotic pressure and Vimentin tension in A549 cells treated with AngII or BK. A A549 cells were transfected with siRNA targeting the mRNA encoding ASC or empty vector. A549 cells were subjected to AngII (10 μM) or BK (10 μM) treatment alone, or co-treatment with AngII or BK and the ASC siRNA, then stained for ASC (FITC) and NLRP3 (TRITC) using antibodies. The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Scale bar:20 μm. B Effects of ASC siRNA on AngII or BK-increased cytoplasmic osmolality (mOsm/kg). C The count rate of PN in A549 cells. D Normalized CFP/FRET ratio in Vimentin tension probe-transfected A549 cells at 15 min after treatment with AngII or BK treatment with the ASC siRNA. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001