Fig.3
The effect of intracellular protein nanoparticles on membrane potential and cytoplasmic calcium. A The membrane potential was measured using the current-clamp method under AngII (10 μM) or BK (10 μM) treatment, and co-treatments of AngII or BK with MCC950 (10 μM) and Ivabradine (50 μM). B Changes in the fluorescence intensity (Ft/F0) of cytoplasmic calcium ions in A549 cells. C The membrane potential was measured using a current-clamp under AngII or BK treatment, and co-treatments of AngII or BK with Nifedipine (30 μM). D Photomicrograph of JC-1 fluorescence mitochondrial used to study the change in mitochondrial membrane potential in the different groups. Scale bar:20 μm. E Changes in fluorescence intensity (Ft/F0) of cytoplasmic calcium ions in the absence of extracellular Ca2+ under AngII or BK treatment, and co-treatment with 2-APB (100 μM), dantrolene (20 μM), or both agents. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001