Fig.4

Effects on DAG-dependent activation of TRPC6 activation and calcium ions under different treatments. A, B Diacylglycerol (DAG) generation was measured under AngII (10 μM) or BK (10 μM) treatment, and AngII or BK with MCC950 (10 μM), Z-VAD-FMK (20 μM), and JK (1 μM). C–G Electrophysiological analysis of whole-cell currents in A549 cells. The voltage-ramp protocol for − 100 mV to 100 mV at a holding potential of 0 mV was given at 3 s intervals. Steady state current densities averaged at potentials of + 80 mV were extracted from the data. Representative current–voltage relationships during application of diacylglycerol (DAG, 100 μM), AngII (10 μM) or BK (10 μM), AngII or BK with MCC950 (10 μM), Z-VAD-FMK (20 μM), and JK (1 μM). Summaries of current densities–voltage relationships at holding potentials of + 80 mV. D–H The relative Fura-2 fluorescence intensity (Ft/F0) of cells under different treatments. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001