Fig.6

Intracellular protein nanoparticle-related osmotic pressure is reduced by effective drugs or their combinations in response to AngII or BK stimuli. A, B Mean values of normalized CFP/FRET ratios of Vimentin tension under the different treatments at 15 min. C Cytoplasmic osmotic pressure in A549 cells were measured after AngII (10 μM) or BK (10 μM) treatment, or with the best effective drug combinations. D Confocal laser microscopy observation of the immunofluorescence of Phalloidin (FITC) and α-tubulin (TRITC) in A549 cells treated with drug combinations. Scale bars, 10 μm. E Immunofluorescence of ASC (TRITC) and NLRP3 (FITC) in A549 cells. The images were generated from the fluorescence inverted microscope after immunofluorescence staining. Scale bar: 20 μm. F–H Changes in fluorescence intensity of cytoplasmic calcium ions (F), chloride ions (G), and sodium ions (H) in A549 cells subjected to AngII or BK, and AngII or BK with drug combinations. Average of ≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001