Fig.8

Changes in the intracellular protein nanoparticle-related osmotic pressure and vimentin tension in response to albumin, NaCl, and drug combinations. A, B Normalized CFP/FRET ratios in vimentin tension of A549 cells were measured with different concentrations of Alb (5, 10, 20, 30, 50 mg/ml) and NaCl (8,16,24,32,40 mM), respectively. C Normalized CFP/FRET ratios of vimentin tension under the different concentrations NaCl and Alb (20 mg/ml) co-treatments. D Normalized CFP/FRET ratios of vimentin tension under Alb treatment, with NaCl, drug combinations. E The cytoplasmic OP values. F The count rate of PN in A549 cells. G, H Changes in the fluorescence intensity of cytoplasmic calcium ions (G) and sodium ions (H) in A549 cells. I, J Normalized TEER values of each group in A549 cells (left) and HPMECs (right), respectively. K, L The membrane potential was measured using the Current-clamp method under Alb treatment, and co-treatments of Alb with NaCl, or drug combinations. Average of≥ 5 experiments ± SEM. ns, p > 0.05, **p < 0.01, ***p < 0.001