Fig. 4

Inhibition of the platelet-induced EMT process by IGBN. a H₂O₂ level in 4T1 cells with different treatments. Scale bar: 200 µm. b The generation of NO in 4T1 cells after different treatments. Scale bar: 7.5 µm. c Confocal fluorescence images of Dil-labeled platelet aggregation with different treatments. Scale bar: 25 µm. d Morphology of 4T1 tumor cells treated with different formulations. Typical cell morphologies were pointed out by dotted squares. Pla: platelets. Scale bar: 50 µm. e Confocal immunofluorescence images of 4T1 cells after treated with different formulations. Green: E-cadherin; blue: nuclei. Scale bar:10 µm. f Schematic illustration of Pt-Arg cascade catalyzes the production of NO within tumor cells. g Western blot analysis of E-cadherin, N-cadherin, and Vimentin expression in 4T1 cells with different treatments (n = 3)